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Michaelis-Menten model rate · formula reference

Assume the stated steady-state model applies; Vmax and v share units, and Km and substrate concentration share units.

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Formula

Assume the stated steady-state model applies; Vmax and v share units, and Km and substrate concentration share units.

v = Vmax[S]/(Km+[S])

Worked example

Under a Michaelis-Menten model, Vmax=149 μmol/min, Km=1 mM, and [S]=18 mM. Calculate v. Round the final result to 4 decimal places if needed. Answer in μmol/min.

Substitute into v=Vmax[S]/(Km+[S]): 149(18)/(1+18).

141.1579 μmol/min

Prerequisites and checking

Algebra and the topic definitions. Keep units and rounding consistent. State the model conditions before applying the formula.

Further reading and review references

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