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Chemistry · Grades 9–12

Chromatography and evidence · Revision sheet

Interpret a supplied chromatogram without claiming one spot proves identity.

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Learn the idea

Separation

Components distribute differently between a mobile phase and a stationary phase.

Rf

In a planar example, Rf = distance traveled by spot / distance traveled by solvent front from the same origin.

Comparison

Rf values are comparable only under matching conditions.

Limits

Co-migration is possible, so matching spots alone need not prove identical substances.

Explain without looking

  1. Why might two analytical methods provide stronger identification than one chromatogram?
  2. What primarily causes separation in chromatography?

Further reading and review references

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Independent educational review required. Grade bands are approximate; these are not officially aligned curricula.